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3D Printed Polylactic Acid Well-Plate for Multi-enzyme Immobilization
Elena Gkantzou1, Anastasia Skonta2, Andreas-Georgios Vasios2
1Laboratory of Biotechnology, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, Greece. e.gkantzou@uoi.gr.
Methods in Molecular Biology (Clifton, N.J.)
|June 10, 2022
Summary
This study presents a protocol for modifying 3D printed polylactic acid (PLA) well-plates to co-immobilize multiple enzymes for cascade reactions. The method enables customized scaffolds for enhanced biocatalysis.
Area of Science:
- Biotechnology
- Materials Science
- Biochemistry
Background:
- 3D printing is increasingly used in biological sciences to create custom scaffolds for biomolecules, cells, or enzymes.
- Enzyme immobilization is crucial for biocatalysis, enabling enzyme reuse and stability.
Purpose of the Study:
- To develop and present a protocol for surface modification of 3D printed polylactic acid (PLA) well-plates.
- To enable co-immobilization of multiple enzymes for cascade reactions on these modified well-plates.
Main Methods:
- Designing and 3D printing polylactic acid (PLA) well-plates.
- Surface modification of PLA well-plates using chitosan coating.
- Covalent immobilization of β-glucosidase, glucose oxidase, and peroxidase using glutaraldehyde cross-linking.
Main Results:
- A detailed protocol for surface modification and enzyme co-immobilization on 3D printed PLA well-plates was successfully developed.
- The immobilized enzymes demonstrated measurable catalytic activity, indicating the system's performance for cascade reactions.
Conclusions:
- The developed protocol offers a method for creating customized enzyme-hosting scaffolds using 3D printing.
- This approach facilitates the development of multi-enzyme systems for efficient cascade reactions in biological applications.
