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Related Experiment Video

Updated: Sep 20, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Recent advances in isobaric labeling and applications in quantitative proteomics.

Michael K Sivanich1, Ting-Jia Gu2, Dylan Nicholas Tabang1

  • 1Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin, USA.

Proteomics
|June 10, 2022
PubMed
Summary

Isobaric tagging in mass spectrometry (MS) enhances quantitative proteomics. This review covers advanced isobaric tags, improved quantitation, and diverse applications for biomarker discovery and structural biology.

Keywords:
isobaric tagsisotopic labelingmass spectrometryprotein quantitationquantitative proteomicssystems biology

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Area of Science:

  • Proteomics
  • Analytical Chemistry
  • Biochemistry

Background:

  • Mass spectrometry (MS) is a leading quantitative proteomic technique.
  • Liquid chromatography-mass spectrometry (LC-MS) quantifies proteins and peptides in biological samples.
  • Chemical isotopic labeling methods enable accurate quantification.

Purpose of the Study:

  • To review isobaric tagging techniques in mass spectrometry.
  • To discuss recent advancements and applications of isobaric tags.
  • To evaluate limitations and solutions for isobaric tag strategies.

Main Methods:

  • Overview of various isobaric tags.
  • Discussion of complementary ion tags and enhanced quantitation.
  • Exploration of multiplexing and targeted analysis strategies.

Main Results:

  • Isobaric tags enable MS2-based peptide quantification.
  • Recent developments improve sensitivity and multiplexing.
  • Bioinformatic tools and data acquisition methods address limitations.

Conclusions:

  • Isobaric tagging is crucial for quantitative proteomics.
  • Diverse applications include biomarker discovery, structural biology, and single-cell analysis.
  • Ongoing advancements enhance sensitivity, multiplexing, and data analysis.