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Updated: Sep 20, 2025
Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
Published on: December 8, 2023
A novel biorecognition receptor Citropin-A modified impedimetric biosensor for detection of LNCaP prostate cancer
Cemrehan Fedacı1, Hilmiye Deniz Ertuğrul Uygun2, Zihni Onur Uygun3
1Ege University, Faculty of Medicine, Department of Medical Biochemistry, Bornova, İzmir, Turkey.
Abstract:
In this study, Citropin-A (Cit-A) as a biorecognition receptor was used for the first time to develop electrochemical impedance spectroscopy (EIS) based biosensor for the detection of Lymph Node Carcinoma of the Prostate (LNCaP) cancer cells. The biosensor was engineered by modification of a gold electrode (AuE) with cysteamine (Cys), Poliamidoamin (PAMAM (G4)) dendrimers, avidin, and biotinylated Cit-A, respectively. The detection time of the LNCaP cells was determined as 300 s by chronoimpedance (CI). Chronoimpedance also provided an exact detection time to avoid non-specific adsorptions. The biosensor showed good linearity between 1500 cells/L and 12000 cells/L, limit of detection (LOD), and limit of quantification (LOQ) values were 518 cells/L and 1570 cells/L, respectively.
