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Small EPIDERMAL PATTERNING FACTOR-LIKE2 peptides regulate awn development in rice.

Luling Xiong1, Yingyong Huang1, Zupei Liu1

  • 1Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, China.

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Summary
This summary is machine-generated.

Researchers investigated EPIDERMAL PATTERNING FACTOR (EPF) and EPF-LIKE (EPFL) genes in rice using CRISPR/Cas9. They found OsEPFL2 disruption affects awns and grain size, suggesting selection during rice domestication.

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Area of Science:

  • Plant biology
  • Genetics
  • Agricultural science

Background:

  • The EPIDERMAL PATTERNING FACTOR (EPF) and EPF-LIKE (EPFL) peptide families regulate plant development, but their roles in rice (Oryza sativa) are not fully understood.
  • Understanding these peptide functions is crucial for improving rice traits.

Purpose of the Study:

  • To systematically investigate the functions of 11 EPF/EPFL genes in rice using CRISPR/Cas9 technology.
  • To identify specific EPFL genes involved in rice development and domestication.

Main Methods:

  • Utilized clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) for gene knockout in the rice cultivar Kasalath.
  • Performed phenotypic analysis, gene expression studies (in young panicles), haplotype analysis, nucleotide diversity, and genetic differentiation analyses.

Main Results:

  • Most OsEPF/EPFL gene knockouts showed no significant phenotypic changes.
  • Disruption of OsEPFL2 resulted in altered awn length (short or absent) and reduced grain size.
  • OsEPFL2 expression is prominent in young panicles, correlating with its role in awn and grain development.
  • Haplotype analysis revealed six major OsEPFL2 haplotypes, with evidence of positive selection during rice domestication.

Conclusions:

  • OsEPFL2 plays a significant role in regulating rice awn and grain development.
  • Different EPF/EPFL family members have undergone independent selection for similar functions during rice domestication.
  • This study provides insights into the genetic basis of rice domestication and potential targets for crop improvement.