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Updated: Sep 8, 2025

Sequential Salt Extractions for the Analysis of Bulk Chromatin Binding Properties of Chromatin Modifying Complexes
Published on: October 2, 2017
NUCKS1 is a highly modified, chromatin-associated protein involved in a diverse set of biological and
Anne Carine Østvold1, Kirsten Grundt1, Claudia Wiese2
1Institute of Basic Medical Science, Department of Biochemistry, University of Oslo, P.O box 1110 Blindern, 0317 Oslo, Norway.
Abstract:
The Nuclear Casein and Cyclin-dependent Kinase Substrate 1 (NUCKS1) protein is highly conserved in vertebrates, predominantly localized to the nucleus and one of the most heavily modified proteins in the human proteome. NUCKS1 expression is high in stem cells and the brain, developmentally regulated in mice and associated with several diverse malignancies in humans, including cancer, metabolic syndrome and Parkinson's disease. NUCKS1 function has been linked to modulating chromatin architecture and transcription, DNA repair and cell cycle regulation. In this review, we summarize and discuss the published information on NUCKS1 and highlight the questions that remain to be addressed to better understand the complex biology of this multifaceted protein.
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