Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Homologous Recombination02:31

Homologous Recombination

62.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
62.7K
Homologous Recombination02:31

Homologous Recombination

6.2K
6.2K
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

6.3K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
6.3K
DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

10.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
10.0K
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

3.0K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
3.0K
Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.9K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CST complex promotes second-strand synthesis in break-induced replication.

Nature structural & molecular biology·2026
Same author

FBH1 and RAD54L directly interact and cooperate to drive replication fork reversal.

bioRxiv : the preprint server for biology·2026
Same author

Multifaceted roles of PDS5B in RAD51-dependent homology-directed DNA repair and replication fork protection.

Nature communications·2026
Same author

Structural insight into how RAD51 paralog exchange regulates RAD51 filament formation.

Nature structural & molecular biology·2026
Same author

Resolution of R-loops and transcription-replication conflicts by SETX-BRCA1-BARD1 complex.

Nature structural & molecular biology·2026
Same author

Cryo-EM structures of UBA6 reveal mechanisms of E1-E2 specificity and dual FAT10/ubiquitin thioester transfer.

Nature communications·2026

Related Experiment Video

Updated: Jan 17, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.0K

Phosphoregulation of RAD51AP1 function in homology-directed repair.

Neelam Sharma1, Mollie Uhrig1,2, Youngho Kwon3

  • 1Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins CO, USA.

Biorxiv : the Preprint Server for Biology
|September 18, 2025
PubMed
Summary

Phosphorylation regulates RAD51AP1

Keywords:
CDK1/2DNA bindingHomology-directed repairRAD51AP1cell cycle

More Related Videos

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.5K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.2K

Related Experiment Videos

Last Updated: Jan 17, 2026

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

11.0K
Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
06:24

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Published on: February 13, 2019

8.5K
Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

2.2K

Area of Science:

  • Molecular Biology
  • DNA Repair Mechanisms
  • Cancer Research

Background:

  • Homology-directed DNA repair (HDR) is crucial for maintaining genome stability and suppressing tumors.
  • RAD51AP1, a protein interacting with RAD51, plays a role in HDR and its overexpression correlates with poor cancer prognosis.

Purpose of the Study:

  • To investigate the role of phosphorylation in regulating RAD51AP1 activity.
  • To elucidate the mechanistic insights into RAD51AP1 regulation by cyclin-dependent kinases (CDKs).

Main Methods:

  • Site-directed mutagenesis to create RAD51AP1 S277/282A and S277/282D mutants.
  • Electrophoretic mobility shift assays (EMSAs) to assess DNA binding.
  • In vitro D-loop formation assays.
  • Cellular toxicity and DNA replication assays.

Main Results:

  • RAD51AP1 mutants with S277/282A substitutions showed enhanced D-loop formation and avid DNA binding compared to wild-type.
  • Conversely, the phosphomimetic S277/282D mutant failed to rescue RAD51AP1 deficiency in cellular assays, unlike the S277/282A mutant.
  • RAD51AP1-S277 was identified as a CDK2 target, suggesting a regulatory role for CDK-mediated phosphorylation.

Conclusions:

  • Phosphorylation at S277/282 regulates RAD51AP1 activity, influencing its DNA binding and function in homology-directed DNA repair.
  • CDK2-mediated phosphorylation of RAD51AP1 is proposed to ensure its flexibility for dynamic participation in HDR.
  • These findings offer new mechanistic understanding of RAD51AP1 regulation by CDKs in DNA repair pathways.