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Related Experiment Video

Updated: Sep 8, 2025

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

A red light-responsive photoswitch for deep tissue optogenetics.

Yuto Kuwasaki¹, Kazushi Suzuki¹, Gaigai Yu¹

¹Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan.

Nature Biotechnology

|June 13, 2022

Summary

This summary is machine-generated.

Researchers developed MagRed, a novel red light-activated photoswitch for optogenetics. This tool enables precise control of biological processes deep within mammalian tissues, advancing in vivo research capabilities.

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Last Updated: Sep 8, 2025

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

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Published on: August 4, 2021

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Building a Simple and Versatile Illumination System for Optogenetic Experiments

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Published on: January 12, 2021

Area of Science:

Optogenetics
Molecular Biology
Biochemistry

Background:

Red light offers advantages for deep tissue penetration and low phototoxicity in optogenetics.
Development of red light-activated optogenetic tools remains limited, hindering in vivo applications.

Purpose of the Study:

To engineer a novel red light-activatable photoswitch, named MagRed.
To demonstrate MagRed's utility for controlling gene expression and DNA recombination in mammalian tissues.

Main Methods:

Designed MagRed by combining a red light-absorbing bacterial phytochrome with a mammalian endogenous chromophore (biliverdin) and an engineered photo-state-specific binder.
Utilized Affibody library selection to develop the photo-state-specific binder.
Fused MagRed components to split-proteins to create light-inducible systems.

Main Results:

MagRed successfully triggered split-protein assembly upon red light illumination.
Developed a red light-activatable Cre recombinase enabling deep-tissue DNA recombination.
Created CRISPR-Cas9-based transcriptional regulators achieving up to 378-fold gene induction.

Conclusions:

MagRed is a functional red light-activatable photoswitch suitable for deep-tissue optogenetics.
The developed tools facilitate precise, light-inducible control of genetic processes in vivo.
MagRed broadens the scope of optogenetic applications in various biological research areas.