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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

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In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Identification of Circular RNAs using RNA Sequencing
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Methodological considerations for circulating long noncoding RNA quantification.

David de Gonzalo-Calvo1, Miron Sopić2, Yvan Devaux3

  • 1Translational Research in Respiratory Medicine, University Hospital Arnau de Vilanova and Santa Maria, IRBLleida, Lleida, Spain; CIBER of Respiratory Diseases (CIBERES), Institute of Health Carlos III, Madrid, Spain.

Trends in Molecular Medicine
|June 14, 2022
PubMed
Summary
This summary is machine-generated.

Long noncoding RNA (lncRNA) research has advanced significantly, yet clinical applications remain limited. This review highlights technical challenges hindering lncRNA

Keywords:
RT-qPCRbiomarkerlong noncoding RNAmethods

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Area of Science:

  • Molecular Biology
  • Genomics
  • Biochemistry

Background:

  • Extensive investment in long noncoding RNA (lncRNA) research over the past decade.
  • lncRNAs play crucial roles in gene regulation and cellular processes.
  • Despite progress, lncRNAs are not yet integrated into routine clinical practice.

Purpose of the Study:

  • To identify and emphasize the key technical challenges in the field of lncRNA research.
  • To stimulate discussion and foster the development of new consensus and guidelines for lncRNA studies.
  • To bridge the gap between lncRNA discovery and clinical utility.

Main Methods:

  • Review and synthesis of current literature on lncRNA research methodologies.
  • Analysis of technical hurdles in lncRNA detection, characterization, and functional validation.
  • Identification of areas requiring standardization and technological innovation.

Main Results:

  • Significant technical challenges impede the translation of lncRNA findings into clinical applications.
  • Key challenges include specificity in detection, functional validation, and delivery methods.
  • Lack of standardized protocols and robust validation methods hinders reliable lncRNA assessment.

Conclusions:

  • Addressing the identified technical challenges is critical for advancing lncRNA research.
  • Development of new consensus and standardized guidelines is necessary for reproducible and clinically relevant lncRNA studies.
  • Overcoming these obstacles will facilitate the clinical integration of lncRNAs as diagnostic or therapeutic agents.