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MiR-335 promotes corneal neovascularization by Targeting EGFR.

Jingjing Qian1, Junbo Yu2, Xi Zhu1

  • 1Department of Ophthalmology, Affiliated Hospital of Nantong University, No. 20 Xisi Road, Nantong, 226001, Jiangsu Province, China.

BMC Ophthalmology
|June 14, 2022
PubMed
Summary

MicroRNA-335 (miR-335) promotes corneal neovascularization (CRNV) by targeting the epidermal growth factor receptor (EGFR). This study clarifies miR-335

Keywords:
AngiogenesisCorneal neovascularizationEGFRHuman umbilical vein endothelial cellsmiR-335

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Angiogenesis Research

Background:

  • Corneal neovascularization (CRNV) poses a significant threat to vision.
  • MicroRNA-335 (miR-335) is known to promote angiogenesis.
  • The specific role of miR-335 in CRNV progression was previously unclear.

Purpose of the Study:

  • To investigate the role of miR-335 in CRNV.
  • To elucidate the underlying molecular mechanism of miR-335 in CRNV.
  • To determine the relationship between miR-335 and the epidermal growth factor receptor (EGFR) in CRNV.

Main Methods:

  • Quantitative real-time PCR to measure miR-335 expression in CRNV rat models and human umbilical vein endothelial cells (HUVECs).
  • Wound healing and tube formation assays to assess the functional impact of miR-335.
  • Dual-luciferase reporter gene assay to identify miR-335 targets.
  • Cell Counting Kit-8 and rescue assays to evaluate the miR-335/EGFR interaction in HUVEC migration and angiogenesis.

Main Results:

  • miR-335 knockdown inhibited HUVEC migration and angiogenesis, while overexpression enhanced these processes.
  • miR-335 was found to directly interact with and negatively regulate EGFR expression.
  • Rescue assays confirmed that miR-335 influences HUVEC migration and angiogenesis via EGFR.

Conclusions:

  • The study confirms that miR-335 facilitates CRNV progression.
  • miR-335 targets EGFR, playing a key role in the development of CRNV.
  • These findings highlight miR-335 as a potential therapeutic target for CRNV.