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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

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Measurement of 3-Dimensional cAMP Distributions in Living Cells using 4-Dimensional x, y, z, and λ Hyperspectral FRET Imaging and Analysis

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Unmixing for ultra-high-plex fluorescence imaging

Archibald Enninful¹, Alev Baysoy¹, Rong Fan^2,3,4,5

¹Department of Biomedical Engineering, Yale University, New Haven, CT, 06520, USA.

Nature Communications

|June 17, 2022

Summary

No abstract available in PubMed .

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