A user-friendly and streamlined protocol for CRISPR/Cas9 genome editing in budding yeast

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Updated: Dec 22, 2025

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

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Daniele Novarina1, Andriana Koutsoumpa1, Andreas Milias-Argeitis1

  • 1Molecular Systems Biology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, the Netherlands.

STAR Protocols
|June 17, 2022
PubMed

Keywords:
CRISPRGeneticsModel OrganismsMolecular Biology

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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