Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Karyotyping01:17

Karyotyping

62.4K
Overview
62.4K
Chromosome Structure02:40

Chromosome Structure

23.4K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
23.4K
Lampbrush Chromosomes01:51

Lampbrush Chromosomes

8.0K
In 1882, Flemming observed lampbrush chromosomes (LBC) in salamander eggs. Later in 1892, Rückert observed LBCs in shark egg cells and coined the term "lampbrush chromosomes" because they looked like brushes used to clean kerosene lamps.
LBCs are made up of two pairs of conjugating homologous chromatids. Each chromatid consists of alternatively positioned regions of condensed-inactive chromatin and loosely placed-active side loops, which can be contracted and extended. The loops...
8.0K
Chromatin Packaging01:32

Chromatin Packaging

17.1K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
17.1K
Polytene Chromosomes02:04

Polytene Chromosomes

10.2K
Polytene chromosomes are giant interphase chromosomes with several DNA strands placed side by side. They were discovered in the year 1881 by Balbiani in salivary glands, intestine, muscles, malpighian tubules, and hypoderm of larvae Chironomus plumosus. Hence, these are also called "Salivary gland chromosomes." These are found in insects of the order Diptera and Collembola; in certain organs of mammals; and synergids, antipodes of flowering plants. Polytene chromosomes are also...
10.2K
Duplication of Chromatin Structure02:05

Duplication of Chromatin Structure

5.7K
The process of chromosome duplication during cell division requires genome-wide disruption and re-assembly of chromatin. The chromatin structure must be accurately inherited, reassembled, and maintained in the daughter cells to ensure lineage propagation.
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
5.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Increased copy number of mitochondrial DNA predicts poor prognosis of esophageal squamous cell carcinoma.

Oncology letters·2018
Same author

Del17p does not always significantly influence the survival of B-cell chronic lymphoproliferative disorders.

Oncotarget·2018
Same author

Processing bulk natural wood into a high-performance structural material.

Nature·2018
Same author

Retrospective study of paraneoplastic neurological syndromes in a Chinese Han population from Shandong, East China.

The International journal of neuroscience·2018
Same author

Genetics of Magnesium Disorders.

Kidney diseases (Basel, Switzerland)·2018
Same author

Efficacy of Rituximab for Patients with Chronic Lymphocytic Leukemia.

Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae·2018

Related Experiment Video

Updated: Sep 7, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.6K

SnapShot: Chromosome organization.

Rachel Patton McCord1, Yang Xu2, Heng Li1

  • 1Department of Biochemistry & Cellular and Molecular Biology, University of Tennessee Knoxville, Knoxville, TN 37996, USA.

Molecular Cell
|June 17, 2022
PubMed
Summary
This summary is machine-generated.

Higher eukaryotic chromosomes fold into complex structures like loop extrusion domains and territories. Researchers use microscopy and sequencing to map these structures and their interactions within the nucleus.

More Related Videos

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

3.6K
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

409.6K

Related Experiment Videos

Last Updated: Sep 7, 2025

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C
09:32

Deciphering High-Resolution 3D Chromatin Organization via Capture Hi-C

Published on: October 14, 2022

3.6K
Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates
09:13

Author Spotlight: Getting an A with the 3Cs: Chromosome Conformation Capture for Undergraduates

Published on: May 12, 2023

3.6K
Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.
22:27

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

Published on: May 6, 2010

409.6K

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Eukaryotic chromosomes exhibit hierarchical folding into distinct structural layers, including loop extrusion domains, spatial compartments, and chromosome territories.
  • These structures are crucial for genome organization and function, and they interact with nuclear components like the nuclear lamina.

Purpose of the Study:

  • To explore the multi-scale organization of chromosomes in higher eukaryotes.
  • To investigate the interplay between chromosomal structures and nuclear architecture.

Main Methods:

  • Utilizing high-resolution microscopy to determine chromosome positions within the nuclear space of individual cells.
  • Employing sequencing-based contact capture techniques to quantify inter-region contact frequencies across different structural layers.

Main Results:

  • Microscopy provides insights into the spatial arrangement of chromosomes.
  • Contact capture methods reveal the frequency and patterns of DNA interactions within the nucleus.
  • Integration of these methods allows for a comprehensive view of chromosome folding and nuclear organization.

Conclusions:

  • The multi-scale folding of eukaryotic chromosomes is a fundamental aspect of nuclear organization.
  • Combining spatial and contact-based methods offers a powerful approach to dissecting chromosome structure and function.
  • Understanding chromosome folding is key to comprehending genome regulation and cellular processes.