Optimizing rat and human blood cells sampling for in silico morphometric analysis

Area of Science:

• Hematology
• Biomedical Imaging
• Histology

Background:

• Morphometric Parameters of the Blood Cells (MPBC) are crucial for diagnosing mental and metabolic diseases.
• While manual and computational methods exist for diagnosis, pre-analytical sample handling is under-evaluated.
• Reliable clinical diagnosis depends on robust sample processing and data analysis.

Purpose of the Study:

• To improve blood smear preservation using a histological resin.
• To provide detailed morphometric descriptions of white blood cells.
• To establish reference intervals for white blood cells in male Wistar rats.

Main Methods:

• Blood smears from male Wistar rats and adult men were collected and preserved with a toluene-based synthetic resin.
• In silico image analysis evaluated the integrity of Wright-stained cells.
• Reference intervals were established using cell counting and segmentation algorithms.

Main Results:

• The histological resin significantly improved the conservation of blood cell populations.
• Morphologic features of white blood cells, including nucleus/cytoplasmic shape and granule presence, were clearly identified.
• The resin facilitated in silico MPBC measurements by enhancing cell preservation.

Conclusions:

• Histological resin offers a practical approach for preserving blood smears.
• This method supports detailed morphometric analysis and establishes reference intervals.
• The resin-based technique can improve efficiency in sample handling for MPBC measurements in various species.