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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Related Experiment Video

Updated: Sep 7, 2025

Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling
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Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling

Published on: October 28, 2014

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Single polysome analysis of mRNP.

Byungju Kim1, Yeonkyoung Park2, Hyun Jung Hwang2

  • 1Department of Physics, Pohang University of Science & Technology (POSTECH), Pohang, 37673, South Korea.

Biochemical and Biophysical Research Communications
|June 18, 2022
PubMed
Summary
This summary is machine-generated.

The closed-loop model of eukaryotic translation may be transient. Researchers found limited colocalization of key proteins, suggesting mRNA circularization is fleeting during translation.

Keywords:
Closed-loop modelPABP(PABPC1)Poly(A) tailPolysomeTranslationmRNA

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Analysis of Translation in the Developing Mouse Brain using Polysome Profiling
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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Eukaryotic translation converts genetic information into proteins.
  • A model proposes mRNA circularization between the 3' poly(A) tail and 5' cap enhances translation.
  • This closed-loop structure involves poly(A)-binding proteins (PABPs) and eukaryotic initiation factor 4E (eIF4E).

Purpose of the Study:

  • To investigate the transient nature of mRNA circularization during eukaryotic translation.
  • To determine the colocalization of key translation factors on individual mRNAs.

Main Methods:

  • Single polysome analysis was employed.
  • The colocalization of eIF4E, eukaryotic initiation factor 4G (eIF4G), and PABP on individual mRNAs was analyzed.
  • Analysis was performed on both polysomal and subpolysomal fractions.

Main Results:

  • The three proteins (eIF4E, eIF4G, PABP) showed minimal coexistence on individual mRNAs.
  • This lack of colocalization was observed in both polysomal and subpolysomal fractions.
  • The results imply that the proposed closed-loop mRNA structure is transient during translation.

Conclusions:

  • The direct observation of mRNA circularization during translation remains elusive.
  • The closed-loop model of translation initiation may involve transient interactions.
  • Further research is needed to fully elucidate the dynamics of mRNA structure in translation.