Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

High-resolution nanopore peptide sensing, profiling and sequence assembly.

Nature nanotechnology·2026

Same author

Single-Molecule Identification of Tea Polyphenols and Their Interactions with Metal Ions Using an Engineered Nanopore.

Nano letters·2026

Same author

Nanopore Identification of Biogenic Amines and Its Applications in Food Freshness Evaluation.

ACS nano·2026

Same author

Nanopore profiling and structure determination of oligosaccharides by fragmentation.

Science advances·2026

Same author

Nanopore Analysis of Malto-oligosaccharides from Amylose, Amylopectin, and Cyclodextrin Hydrolysates.

Nano letters·2025

Same author

Chiral Discrimination of all Proteinogenic Amino Acid Enantiomers by Nanopore Sensing.

Angewandte Chemie (International ed. in English)·2025

Same journal

Efficient Syngas Photoproduction Enabled by Electronic Engineering of Co-Immobilized Imine COFs.

Angewandte Chemie (International ed. in English)·2026

Same journal

Pathway Controlled Phase Separation of Minimal Building Blocks Utilizing a Dissociative Chemical Transformation.

Angewandte Chemie (International ed. in English)·2026

Same journal

Interaction Hierarchy and Polymorphic Structure-Property Dynamics in Luminescent Molecular Crystals.

Angewandte Chemie (International ed. in English)·2026

Same journal

The Role of Zn-Hf Site Proximity and Oxygen Vacancies for Methanol Formation Over ZnHfO<sub>x</sub> Catalysts Under CO<sub>2</sub> Hydrogenation Conditions.

Angewandte Chemie (International ed. in English)·2026

Same journal

Breaking the Linear Scaling Relationship: Bioinspired Electronic Coupling in S-Bridged Fe-Fe Dual Sites for Efficient Oxygen Reduction.

Angewandte Chemie (International ed. in English)·2026

Same journal

Programming Bio-Bio Electronic Interfaces for Light-Driven Interspecies Electron Transfer.

Angewandte Chemie (International ed. in English)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Sep 7, 2025

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

A Nanopore-Based Saccharide Sensor.

Shanyu Zhang^1,2, Zhenyuan Cao^1,2, Pingping Fan^1,2

¹State Key Laboratory of Analytical Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, 210023, Nanjing, China.

Angewandte Chemie (International Ed. in English)

|June 19, 2022

Summary

This summary is machine-generated.

This study demonstrates how engineered nanopores can identify different types of saccharides, even distinguishing between similar structures. A machine learning algorithm achieved high accuracy, paving the way for nanopore saccharide sequencing.

Keywords:

Boronic Acid Nanopores Saccharides Sequencing Single-Molecule Studies

More Related Videos

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Related Experiment Videos

Last Updated: Sep 7, 2025

A Polyaniline-based Sensor of Nucleic Acids

A Polyaniline-based Sensor of Nucleic Acids

Published on: November 1, 2016

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors

Published on: November 20, 2013

Area of Science:

Biochemistry
Nanotechnology
Analytical Chemistry

Background:

Saccharides are crucial for cellular functions but difficult to identify due to complex structures.
Nanopore technology offers a sensitive method for single-molecule analysis, capable of detecting subtle structural variations.

Purpose of the Study:

To engineer a nanopore capable of distinguishing between various saccharide types.
To develop a machine learning algorithm for automated saccharide identification using nanopore data.

Main Methods:

A hetero-octameric Mycobacterium smegmatis porin A nanopore functionalized with phenylboronic acid was constructed.
The nanopore's ability to differentiate nine distinct monosaccharides and epimers was tested.
A machine learning algorithm was developed for event classification.

Main Results:

The engineered nanopore successfully identified nine types of monosaccharides, including glucose and fructose.
The system demonstrated sensitivity to minor structural differences, distinguishing between saccharide epimers.
The machine learning algorithm achieved a high accuracy score of 0.96 for automatic event classification.

Conclusions:

Engineered nanopores with phenylboronic acid provide a robust platform for saccharide identification.
This approach offers a sensitive and accurate method for analyzing complex carbohydrate structures.
The developed strategy shows promise for advancing nanopore-based saccharide sequencing and analysis.