Bacterial Peroxidase on Electrochemically Reduced Graphene Oxide for Highly Sensitive H₂ O₂ Detection

Area of Science:

• Biotechnology
• Electrochemistry
• Materials Science

Background:

• Peroxidase enzymes are crucial for developing sensitive electrochemical sensors.
• Existing sensors often face limitations in sensitivity and stability.

Purpose of the Study:

• To immobilize Bacillus s. peroxidase (BsDyP) onto electrochemically reduced graphene oxide (ERGO) for enhanced biosensing.
• To characterize and evaluate the performance of the developed BsDyP-ERGO biosensor.

Main Methods:

• Immobilization of BsDyP on ERGO deposited on Indium Tin Oxide (ITO) and Polyethylene Terephthalate (PET) layers.
• Characterization using XRD, SEM, AFM, FT-IR, and Raman spectroscopy.
• Electrochemical performance evaluation for hydrogen peroxide (H₂O₂) detection.

Main Results:

• Successful immobilization of BsDyP on ERGO/ITO/PET, confirmed by various characterization techniques.
• The BsDyP-ERGO electrode demonstrated superior electrochemical response compared to horseradish peroxidase-based electrodes.
• Achieved high sensitivity for H₂O₂ detection in the nanomolar range (LOD 32 nM) with excellent linearity (0.05–280 μM).
• The bioelectrode exhibited mechanical robustness, a wide pH activity range (3.5–6), and storage stability for 8 weeks.

Conclusions:

• The BsDyP-ERGO/ITO/PET bioelectrode represents a significant advancement in electrochemical sensing.
• This novel biosensor offers a promising platform for sensitive and stable quantitative detection of hydrogen peroxide.