The esterified lentinan bilayer nanofibrous membrane for promoting wound healing

Host reactions following implantation of biomaterials, especially the macrophages' responses could significantly affect the wound healing process. Therefore, it is meaningful to develop immunostimulatory active wound dressing to accelerate wound healing. In this study, lentinan (LNT) was esterified with different carboxylic acids, and the bilayer nanofibrous membrane (BilayerM) based on the esterified LNT was designed. BilayerM exhibited good water absorption, red blood cells (RBC), platelets, and fibronectin adhesion abilities. The ELISA results indicated that BilayerM stimulated macrophages to secrete pro-inflammatory and pro-regenerative cytokines. Moreover, cell migration results showed that BilayerM promoted the migration and proliferation of NIH3T3 and HUVECs. The wound healing efficacy studies demonstrated that BilayerM significantly accelerated the wound healing rate in a full-thickness skin defect model. Therefore, these results indicate that this bioactive dressing is a hopeful candidate for clinical treatment of cutaneous wounds.