Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ultrasound II: Endoscopic Ultrasound and FibroScan01:25

Ultrasound II: Endoscopic Ultrasound and FibroScan

197
Endoscopic Ultrasound (EUS) and FibroScan are valuable diagnostic tools in gastroenterology and hepatology, each with specific applications and techniques.
Endoscopic Ultrasound (EUS):
197

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From the Editor's Desk...

Journal of hepatology·2026
Same author

From the Editor's Desk….

Journal of hepatology·2026
Same author

From the Editor's Desk...

Journal of hepatology·2026
Same author

Implementing sustainable liver health in Europe: a second EASL-Lancet Commission.

Lancet (London, England)·2026
Same author

Accurate detection of cholangiocarcinoma in primary sclerosing cholangitis using DNA methylation biomarkers in bile and plasma.

JHEP reports : innovation in hepatology·2026
Same author

Mucosal-Associated Invariant T Cells Drive Bile Duct Inflammation.

Cellular and molecular gastroenterology and hepatology·2026

Related Experiment Video

Updated: Sep 7, 2025

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis
06:46

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Published on: February 25, 2022

2.0K

Spatial transcriptomics identifies enriched gene expression and cell types in human liver fibrosis.

Brian K Chung1,2,3, Jonas Øgaard1,2, Henrik Mikael Reims4

  • 1Norwegian PSC Research Center, Oslo University Hospital, Rikshospitalet, Oslo, Norway.

Hepatology Communications
|June 21, 2022
PubMed
Summary

Spatial transcriptomics precisely maps cell types within fibrotic liver regions, revealing unique gene expression patterns. This approach aids in identifying potential antifibrotic targets for liver fibrosis and cirrhosis.

More Related Videos

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

7.8K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K

Related Experiment Videos

Last Updated: Sep 7, 2025

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis
06:46

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Published on: February 25, 2022

2.0K
Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

7.8K
Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K

Area of Science:

  • Hepatology
  • Molecular Biology
  • Genomics

Background:

  • Liver fibrosis and cirrhosis lack effective treatments, posing a significant unmet medical need.
  • Single-cell RNA sequencing (scRNAseq) has identified cell types in cirrhotic livers but lacks precise microanatomical localization.
  • Understanding cell distribution in fibrotic liver regions is crucial for developing targeted therapies.

Purpose of the Study:

  • To assess if fibrotic liver regions harbor distinct, enriched cell types.
  • To utilize whole-tissue spatial transcriptomics combined with scRNAseq and gene deconvolution to localize cell types in cirrhotic liver explants.
  • To demonstrate the utility of spatial transcriptomics in refining cell lineage localization for antifibrotic target discovery.

Main Methods:

  • Analysis of cirrhotic liver explants (n=8) from patients with end-stage liver disease using whole-tissue spatial transcriptomics.
  • Integration with scRNAseq and gene deconvolution analysis to enumerate cell states.
  • Correlation of spatial transcriptomics data with conventional histology for parenchymal and fibrotic regions.

Main Results:

  • Spatial transcriptomics accurately identified and localized distinct gene expression areas corresponding to histological parenchymal and fibrotic regions.
  • Fibrotic liver regions showed significantly higher frequencies of specific mesenchymal cells, monocytes, Kupffer cells, T cells, and B cells compared to parenchymal areas.
  • Gene expression patterns in spatial transcriptomes were highly congruent with the location of parenchymal and fibrotic liver tissues.

Conclusions:

  • Spatial transcriptomes of parenchymal and fibrotic liver regions exhibit unique gene content in cirrhosis.
  • Spatial transcriptomics, coupled with other RNA sequencing methods, can precisely localize cell lineages within the cirrhotic liver.
  • This approach provides a powerful proof of concept for identifying novel antifibrotic therapeutic targets.