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Updated: Sep 7, 2025

In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

In Vivo Study of Human Endothelial-Pericyte Interaction Using the Matrix Gel Plug Assay in Mouse

Published on: December 19, 2016

Bioink derived from human placenta supporting angiogenesis.

Yongchao Duan^1,2, Wenhui Huang^1,2, Bo Zhan^1,2

¹State Key Laboratory of Membrane Biology and State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.

Biomedical Materials (Bristol, England)

|June 22, 2022

Summary

This summary is machine-generated.

Researchers developed a novel human placental extracellular matrix-derived bioink for 3D bioprinting. This bioink demonstrates excellent printability and supports cell growth and blood vessel formation in vitro and in vivo.

Keywords:

3D printing ECM angiogenesis bioink human placenta

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Last Updated: Sep 7, 2025

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Published on: September 7, 2017

Area of Science:

Biotechnology
Regenerative Medicine
Materials Science

Background:

Advanced tissue engineering requires biomaterials that mimic the native extracellular matrix (ECM) for creating complex tissue constructs.
Developing bioinks with both high printability and bioactivity is crucial for successful 3D bioprinting of functional tissues, including vascular networks.

Purpose of the Study:

To develop and characterize a novel human placental extracellular matrix-derived bioink (hp-bioink) for 3D bioprinting applications.
To evaluate the printability and bioactivity of the hp-bioink for tissue engineering, focusing on vascularization.

Main Methods:

Human placenta was decellularized, followed by enzymatic digestion, dialysis, lyophilization, and re-solubilization to create the hp-bioink.
The printability of hp-bioink was assessed at different concentrations (1-5%) for self-standing and embedded printing within hydrogels.
In vitro cell culture with human umbilical vein endothelial cells and in vivo studies in mice were performed to evaluate bioactivity and angiogenic potential.

Main Results:

The developed hp-bioink exhibited suitable properties for 3D printing, with self-standing capabilities at 3-5% concentration and embeddability within hydrogels at 1-2% concentration.
The hp-bioink successfully supported the in vitro assembly of human umbilical vein endothelial cells.
Significant angiogenesis was observed in vivo in mice models, indicating the bioink's potential for promoting blood vessel formation.

Conclusions:

Human placental ECM-derived bioink is a promising biomaterial for 3D bioprinting, offering excellent printability and bioactivity.
This novel bioink facilitates endothelial cell assembly and in vivo angiogenesis, advancing the field of tissue engineering.
The development of hp-bioink expands the range of human-derived biomaterials for bioprinting, accelerating research and therapeutic applications.