Preparation and in vivo bacteriostatic application of PPDO-coated Ag loading TiO₂ nanoparticles

Implant-associated infections limit the clinical application of implants therapy; hence, exploiting strategies to prevent biomaterial-associated infections has become important. Therefore, in this study, a series of poly (p-dioxanone) (PPDO)-coated Ag loading TiO₂ nanoparticles (Ag@TiO₂-PPDO) was synthesized to be applied as bacteriostatic coating materials that could be easily dispersed in organic solvent and coated onto implantable devices via temperate methods such as electrospraying. The lattice parameters of TiO₂ were a = 0.504 nm, b = c = 1.05 nm, alpha = beta = gamma = 90 degree and the size of crystallite was about 13 nm, indicating that part of Ag has been embedded into crystal defects of TiO₂. Both XRD and TEM determinations indicated the successful grating of PPDO on the surface of Ag@TiO₂. Among Ag@TiO₂ nanoparticles with various Ag loading quantities, 12% Ag@TiO₂ nanoparticles exhibited relatively higher grafting efficiency and Ag contents on the surface of grafted composites. In addition, 12% Ag@TiO₂-PPDO exhibited the best bacteriostatic effect in vitro owing to its higher grafted efficiency and relatively short length of PPDO segments. Subsequently, Ag@TiO₂-PPDO was coated on the surface of a poly lactic-co-glycolic acid (PLGA) electrospun membrane via the electrospraying method. Finally, the in vivo bacteriostatic effect of 12% Ag@TiO₂-PPDO coating was verified by implanting 12% Ag@TiO₂-PPDO-coated PLGA membrane into a rat subcutaneously combined with an injection of Staphylococcus aureus at implanting sites.