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Updated: Sep 7, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy
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Two octaves spanning photoacoustic microscopy.

Gianni Nteroli1, Manoj K Dasa2,3, Giulia Messa4

  • 1Applied Optics Group, University of Kent, Canterbury, UK. G.Nteroli@kent.ac.uk.

Scientific Reports
|June 22, 2022
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Summary
This summary is machine-generated.

This study demonstrates a novel Photoacoustic Microscopy instrument using a single, wide-spectrum optical source. It enables in vivo imaging and mapping of multiple endogenous contrast agents in Xenopus laevis tadpoles.

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Area of Science:

  • Biomedical Optics
  • Photoacoustic Imaging
  • Microscopy

Background:

  • Photoacoustic Microscopy (PAM) offers high contrast and resolution for biological tissue imaging.
  • Current PAM systems often require multiple light sources for broad spectral coverage.
  • Mapping endogenous absorbers in vivo is crucial for understanding physiological processes.

Purpose of the Study:

  • To demonstrate a novel Photoacoustic Microscopy instrument driven by a single, ultra-wide spectral range optical source.
  • To develop and validate a new technique for mapping absorbers in biological samples.
  • To perform in vivo hyperspectral imaging and identify multiple endogenous contrast agents.

Main Methods:

  • Utilized a single supercontinuum optical source (475-2400 nm) for Photoacoustic Microscopy.
  • Imaged Xenopus laevis tadpoles in vivo across the entire 2000 nm spectral range.
  • Acquired hyperspectral Photoacoustic images using narrow spectral windows (25 nm bandwidth).
  • Developed a post-processing algorithm to map absorbers based on single-chromophore contribution per voxel.

Main Results:

  • Successfully demonstrated a Photoacoustic Microscopy instrument with over two octaves of spectral coverage from a single source.
  • Generated in vivo hyperspectral Photoacoustic images of Xenopus laevis tadpoles.
  • Produced maps of five endogenous contrast agents: hemoglobin, melanin, collagen, glucose, and lipids.
  • Validated a novel absorber mapping technique based on spectral deconvolution.

Conclusions:

  • The developed single-source, ultra-wide spectral range Photoacoustic Microscopy system is effective for in vivo imaging.
  • The novel absorber mapping technique enables simultaneous visualization of multiple endogenous chromophores.
  • This technology holds significant potential for label-free biomedical research and diagnostics.