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Updated: Sep 7, 2025
Assays for Validating Histone Acetyltransferase Inhibitors
Published on: August 6, 2020
Preparation and Characterization of Chromatin Templates for Histone Methylation Assays
Cuifang Liu1, Jicheng Zhao1, Guohong Li2,3
1National Laboratory of Biomacromolecules, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing, China.
Abstract:
In eukaryotic cells, chromatin plays an important role in gene regulation by controlling the access of the transcription machinery to DNA. In this chapter, we will describe methods for generating different chromatin templates to investigate the impact of histone variants and chromatin structure on histone methyltransferase activities. For this purpose, we take Polycomb Repressive Complex 2 (PRC2) as an example and investigate how its activity on H3K27me3 is regulated by the histone variants H3.3 and H2A.Z and higher-order chromatin structure.
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