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Updated: Sep 7, 2025

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Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
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Development and Validation of Antibodies Targeting Site-Specific Histone Methylation
Lara Zorro Shahidian1, Sylvain Daujat2
1Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), University of Cantabria, Santander, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|June 22, 2022
Abstract:
The development of specific anti-modification antibodies as research tools has revolutionized the way histone methylation is studied. Lack of stringent quality controls, however, led to the development of nonspecific antibodies, compromising their use. In this chapter, we provide a series of protocols that collectively will help those studying histone methylation to develop and thoroughly validate high-end sequence-specific and methylation-dependent antibodies.

