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Related Experiment Video

Updated: Sep 7, 2025

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

Published on: February 9, 2009

Visualizing Influenza A Virus vRNA Replication.

Ya-Fang Chiu^1,2,3, Yi-Wen Huang¹, Chi-Yuan Chen¹

¹Department of Microbiology and Immunology, Chang Gung University, Taoyuan, Taiwan.

Frontiers in Microbiology

|June 23, 2022

Summary

This summary is machine-generated.

Influenza A virus (IAV) replication, visualized using MS2-MCP live-cell imaging, occurs exclusively in apoptotic host cells. This study reveals apoptosis initiation is critical for IAV vRNA replication, highlighting a new therapeutic target.

Keywords:

apoptosis influenza A virus live-cell imaging vRNA viral-host interaction

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Purification and Visualization of Influenza A Viral Ribonucleoprotein Complexes

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Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Published on: January 18, 2018

Area of Science:

Virology
Cell Biology
Molecular Biology

Background:

Influenza A virus (IAV) poses significant public health threats due to recurrent epidemics and pandemics.
Understanding the spatiotemporal dynamics of IAV replication is crucial for developing effective antiviral strategies.

Purpose of the Study:

To adapt and utilize an MS2-MCP live-cell imaging system to visualize IAV vRNA replication in real-time.
To investigate the role of host cell apoptosis in the IAV replication cycle.

Main Methods:

Construction of a reporter plasmid (pHH-PB2-vMSL) encoding MS2 stem-loop (MSL) structures within the IAV PB2 gene.
Transduction of A549 and MDCK cells to express MS2 coat protein (MCP) fused to GFP (MCP-GFPdN).
Live-cell imaging to track fluorescently labeled vRNA puncta during IAV PR8 infection.

Main Results:

MS2-MCP system successfully detected IAV vRNA as fluorescent puncta in live cells.
IAV vRNA replication, indicated by signal intensity increases, was exclusively observed in apoptotic cells.
Replication occurred post-apoptosis initiation and was suppressed by apoptosis inhibitors, but absent in non-apoptotic cells.

Conclusions:

Apoptosis plays a critical and essential role in supporting Influenza A virus vRNA replication.
The MS2-MCP live-cell imaging system is a powerful tool for studying dynamic viral processes in host cells.