Related Experiment Video

Updated: Sep 7, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
08:56

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues

Published on: December 5, 2016

11.0K

Novel insights into the interaction between N6-methyladenosine methylation and noncoding RNAs in musculoskeletal

Juanjuan Han1,2, Hui Kong1, Xueqiang Wang2,3

  • 1College of Kinesiology, Shenyang Sport University, Shenyang, China.

Cell Proliferation
|June 23, 2022
PubMed
Abstract

Insights

N6-methyladenosine (m6A) RNA modification and noncoding RNAs (ncRNAs) interact to regulate musculoskeletal disorders (MSDs). Targeting these interactions offers a promising therapeutic strategy for MSDs like osteoarthritis and osteoporosis.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Musculoskeletal disorders (MSDs) are inflammatory and degenerative diseases with poorly understood molecular mechanisms.
  • Noncoding RNA (ncRNA) N6-methyladenosine (m6A) modification is crucial in the pathophysiology of MSDs.
  • Understanding the interplay between m6A and ncRNAs offers new therapeutic avenues for MSDs.

Purpose of the Study:

  • To review the interaction between m6A RNA methylation and ncRNAs in the molecular regulatory mechanisms of MSDs.
  • To provide insights into the pathophysiological mechanisms of MSDs.
  • To explore ncRNA m6A targeted therapy for MSDs.

Main Methods:

  • A comprehensive literature search was conducted using keywords related to MSDs and RNA modifications.
  • Relevant articles were systematically reviewed and summarized.
  • Key findings on the mutual regulation between m6A and ncRNAs in MSDs were synthesized.

Main Results:

  • Intervertebral disc degeneration (IDD), osteoporosis (OP), osteosarcoma (OS), and osteoarthritis (OA) are common MSDs affecting muscle, bone, and cartilage.
  • The precise pathogenesis of MSDs remains unclear, with no effective treatments currently available.
  • Mutual regulation between m6A and ncRNAs (microRNAs, long ncRNAs, circular RNAs) is evident in MSDs, impacting skeletal homeostasis.

Conclusions:

  • m6A and ncRNAs interact to regulate biological processes implicated in IDD, OP, OS, and OA.
  • This interaction provides novel insights into MSD pathophysiology.
  • Targeting m6A-modified ncRNAs presents a promising therapeutic strategy for MSDs.

More Related Videos

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry08:45

Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry

Published on: April 21, 2022

2.5K
Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents06:51

Systemic Delivery of MicroRNA Using Recombinant Adeno-associated Virus Serotype 9 to Treat Neuromuscular Diseases in Rodents

Published on: August 10, 2018

7.8K

Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.9K
Types of RNA01:20

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
6.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.2K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.7K
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
3.1K