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Related Concept Videos

Microbial Biosensors01:17

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

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Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
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A Novel Prototype Biosensor Array Electrode System for Detecting the Bacterial Pathogen Salmonella typhimurium.

Palaniappan Ramasamy1,2, Gajalakshmi Dakshinamoorthy2,3, Shanmugam Jayashree2,4

  • 1Research and Development Wing, Bharath Institute of Higher Education and Research (BIHER), Sree Balaji Medical College and Hospital (SBMCH), Chromepet, Chennai 600044, Tamil Nadu, India.

Biosensors
|June 23, 2022
PubMed
Summary

A novel biosensor using a dialysis membrane electrode interface effectively detects Salmonella at very low concentrations. This sensitive Salmonella detection system offers a promising tool for microbial pathogen surveillance.

Keywords:
Salmonella speciesbiosensordialysis membrane electrode interface discglassy carbon electrodeimmunosensorsalmonellosis

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Area of Science:

  • Biosensor technology
  • Immunodiagnostics
  • Microbial pathogen detection

Background:

  • Salmonellosis, caused by Salmonella, is a global health concern requiring improved diagnostic methods.
  • Current detection systems often lack the sensitivity or ease of use needed for effective surveillance.
  • There is a continuous need for rapid, sensitive, and user-friendly biosensors for microbial pathogens.

Purpose of the Study:

  • To develop and characterize a novel biosensor for the sensitive detection of Salmonella.
  • To evaluate the performance of a dialysis membrane electrode interface for Salmonella capture and detection.
  • To assess the biosensor's detection limit and specificity against common bacterial contaminants.

Main Methods:

  • A dialysis membrane electrode interface disc was fabricated with immobilized anti-Salmonella antibodies.
  • Salmonella cells were captured by the immobilized antibodies, followed by a sandwich assay using enzyme-conjugated secondary antibodies (HRP).
  • The enzymatic reaction with o-aminophenol substrate generated a measurable current signal detected by a multimeter without a potentiostat.

Main Results:

  • The biosensor demonstrated high sensitivity with a detection limit as low as three Salmonella cells.
  • A linear response was observed across a wide range of Salmonella concentrations (3 to 300,000 cells).
  • The dialysis membrane interface showed superior performance compared to other materials like gelatin, collagen, and agarose, with minimal non-specific binding.

Conclusions:

  • The developed biosensor offers a highly sensitive and effective method for Salmonella detection.
  • The dialysis membrane electrode interface provides a robust platform for biosensor development.
  • This novel system holds significant potential for future applications in microbial pathogen surveillance and diagnostics.