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Equine Oviductal Organoid Generation and Cryopreservation
Riley E Thompson1, Mindy A Meyers1, D N Rao Veeramachaneni2
1Department of Clinical Sciences, Colorado State University, Fort Collins, CO 80523, USA.
Methods and Protocols
|June 23, 2022
Summary
Researchers developed equine oviductal organoids, a 3D cell culture model. This breakthrough offers a new way to study mare reproductive physiology and improve in vitro embryo production in horses.
Area of Science:
- Reproductive Biology
- Stem Cell Biology
- Animal Science
Background:
- Organoids are 3D cell cultures that mimic in vivo conditions.
- Previous oviductal organoids were developed in mice, humans, and cattle.
- Equine oviductal organoids had not been previously reported.
Purpose of the Study:
- To generate and culture equine oviductal organoids.
- To establish a 3D model of the mare oviduct.
- To explore applications in understanding equine reproductive physiology and improving embryo production.
Main Methods:
- Generation and long-term culture of equine oviductal organoids.
- Maintenance through multiple passages and freeze-thawing.
- Characterization of organoid structure, including lumen formation.
Main Results:
- Equine oviductal organoids were successfully generated and cultured for 42 days.
- Organoids exhibited characteristics similar to those reported in other species, forming round cell clusters with a central lumen.
- The model demonstrated stability through passages and freeze-thawing.
Conclusions:
- The development of equine oviductal organoids provides a novel 3D in vitro model for the mare oviduct.
- This model can enhance understanding of equine oviductal physiology, including hormonal regulation.
- Equine oviductal organoids hold potential for advancing in vitro embryo production techniques in equids.
