Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Lines01:16

Cell Lines

8.1K
A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
8.1K
Cell Culture01:21

Cell Culture

18.4K
Most vertebrate cells grow in vitro attached to a substrate as a monolayer, called adherent cultures. The flasks and plates used to grow cells are chemically treated to facilitate cell attachment. However, a few cell types, such as hematopoietic cells, can grow in a suspension. In contrast to adherent cultures, suspension cultures can grow in non-treated cultureware using magnetic stirrers or spinner flasks to agitate the culture media
18.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

LINC01929 mediates breast cancer immunosuppression and is an immunotherapy target.

iScience·2026
Same author

Extracellular vesicles in malignant and normal B lymphocyte growth and development.

Extracellular vesicles and circulating nucleic acids·2026
Same author

THSD4 is a novel mediator of T cell exclusion and anti-PD-1 resistance in hormone receptor-positive breast cancer.

Biomarker research·2025
Same author

Genomic analysis of glaucoma pathogenesis due to gmds mutation in zebrafish.

Experimental eye research·2025
Same author

Identification of lysyl oxidase as an adipocyte-secreted mediator that promotes a partial mesenchymal-to-epithelial transition in MDA-MB-231 cells.

Exploration of targeted anti-tumor therapy·2024
Same author

Extracellular vesicle small RNA cargo discriminates non-cancer donors from pediatric B-lymphoblastic leukemia patients.

Frontiers in oncology·2023
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Sep 6, 2025

Monitoring the Cancer-Immunity Cycle and Exploring Tumor Microenvironment Dynamics
12:19

Monitoring the Cancer-Immunity Cycle and Exploring Tumor Microenvironment Dynamics

Published on: June 7, 2024

1.3K

Culturing Suspension Cancer Cell Lines.

Modeline N Longjohn1, Hong-Dien Phan1, Sherri L Christian2

  • 1Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2022
PubMed
Summary
This summary is machine-generated.

This guide details best practices for working with suspension cell lines, crucial for cancer research. It covers thawing, culturing, and cryopreserving these cells while minimizing contamination risks.

Keywords:
CancerCell cultureCell lineCryopreservationCulture mediaSuspension cells

More Related Videos

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines
07:49

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

2.6K
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
07:01

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Published on: March 1, 2020

10.3K

Related Experiment Videos

Last Updated: Sep 6, 2025

Monitoring the Cancer-Immunity Cycle and Exploring Tumor Microenvironment Dynamics
12:19

Monitoring the Cancer-Immunity Cycle and Exploring Tumor Microenvironment Dynamics

Published on: June 7, 2024

1.3K
Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines
07:49

Author Spotlight: Transmitochondrial Cybrid Generation Using Cancer Cell Lines

Published on: March 17, 2023

2.6K
Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors
07:01

Obtaining Cancer Stem Cell Spheres from Gynecological and Breast Cancer Tumors

Published on: March 1, 2020

10.3K

Area of Science:

  • Cell Biology
  • Biotechnology
  • Cancer Research

Background:

  • Suspension cell lines grow freely in culture media, unlike adherent cells.
  • They mimic circulating cells (e.g., lymphocytes, neutrophils) found in vivo.
  • These cell types are vital for studying blood-related cancers and cellular processes.

Purpose of the Study:

  • To provide comprehensive protocols for handling suspension cell lines.
  • To outline best practices for maintaining cell line integrity and preventing contamination.
  • To support experimental studies utilizing suspension cells in cancer biology and biochemistry.

Main Methods:

  • Detailed protocols for thawing frozen suspension cell lines.
  • Established procedures for routine culturing of suspension cells.
  • Cryopreservation techniques for long-term storage of suspension cell lines.

Main Results:

  • Effective methods for successful cell line thawing and initiation of cultures.
  • Optimized culturing conditions ensuring cell viability and growth.
  • Reliable cryopreservation protocols to maintain cell line characteristics.

Conclusions:

  • Proper techniques are essential for successful suspension cell line culture.
  • Adherence to best practices minimizes contamination risks, ensuring reliable experimental data.
  • These protocols facilitate robust research in cancer biology and related fields.