Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

117
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
117
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

204
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
204
CRISPR01:59

CRISPR

52.8K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.8K
Homologous Recombination02:31

Homologous Recombination

51.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
51.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure-guided development of an electrochemical aptasensor for Salmonella Typhi HlyE antigen detection using in silico and experimental approaches.

Scientific reports·2026
Same author

CRISPR-Cas13a-Powered Electrochemical Biosensor for Label-Free Detection of Chikungunya Virus RNA Using a Ceria-Modified Screen-Printed Carbon Electrode.

ACS sensors·2026
Same author

Performance of Antibody-Detection Tests for Human Melioidosis: A Systematic Review and Meta-analysis.

The Malaysian journal of medical sciences : MJMS·2025
Same author

Isolation and characterization of DNA aptamers against the HlyE antigen of Salmonella Typhi.

Scientific reports·2024
Same author

Isolation and characterization of ssDNA aptamers against BipD antigen of Burkholderia pseudomallei.

Analytical biochemistry·2024
Same author

A portable label-free electrochemical DNA biosensor for rapid detection of <i>Salmonella</i> Typhi.

Analytical methods : advancing methods and applications·2024

Related Experiment Video

Updated: Sep 6, 2025

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

506

CRISPR-Cas Systems-Based Bacterial Detection: A Scoping Review.

Kasturi Selvam1, Mohamad Ahmad Najib1, Muhammad Fazli Khalid1

  • 1Institute for Research in Molecular Medicine (INFORMM), Health Campus, Universiti Sains Malaysia, Kubang Kerian 16150, Kelantan, Malaysia.

Diagnostics (Basel, Switzerland)
|June 24, 2022
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas systems show promise for bacterial detection, with Cas12a being the most utilized enzyme. Further research is needed to evaluate clinical accuracy and diagnostic specificity for nucleic acid-based tests.

Keywords:
CRISPRCas enzymesbacterial infectionsdetectionscoping review

More Related Videos

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.4K

Related Experiment Videos

Last Updated: Sep 6, 2025

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

506
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.4K

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Microbiology

Background:

  • CRISPR-Cas systems are emerging as powerful tools for bacterial detection assays.
  • A comprehensive understanding of current evidence is crucial for advancing this technology.

Purpose of the Study:

  • To map existing evidence on the development and utilization of CRISPR-Cas systems for bacterial detection.
  • To identify trends in Cas enzyme usage, detection strategies, and target selection.

Main Methods:

  • A scoping review was conducted using PubMed, Scopus, and Cochrane Library databases, supplemented by manual searches.
  • 46 studies published before October 2021, focusing on bacterial detection using CRISPR-Cas systems, were included.
  • Risk of bias was assessed using the CASP qualitative checklist.

Main Results:

  • Cas12a (64%) was the most frequently used enzyme, followed by Cas13a (13%) and Cas9 (11%).
  • Trans-cleavage capabilities were exploited in 83% of studies for signal enhancement, with isothermal amplification (66%) and fluorescence detection (67%) being common.
  • Most studies used spiked samples (78%) and identified bacteria from genera like *Staphylococcus*, *Escherichia*, and *Salmonella*.

Conclusions:

  • CRISPR-Cas systems are versatile for detecting bacteria from various genera, with Cas12a dominating current applications.
  • A significant gap exists in evaluating the clinical accuracy, diagnostic sensitivity, and specificity of these systems.
  • Further research is essential to validate amplification-free CRISPR-Cas systems for clinical nucleic acid-based bacterial detection.