Microalgae Peptide-Stabilized Gold Nanoparticles as a Versatile Material for Biomedical Applications

Area of Science:

• Biomaterials Science
• Nanotechnology
• Marine Biotechnology

Background:

• Microalgae peptides offer valuable functional properties for medical and industrial uses.
• Peptide instability in biological settings poses a significant challenge, limiting their applications.
• Carrier systems are crucial for protecting peptides and controlling their release.

Purpose of the Study:

• To investigate gold nanoparticles (AuNPs) as carriers for the microalgae peptide VECYGPNRPQF.
• To evaluate the peptide's ability to stabilize AuNPs in physiological environments.
• To develop an effective peptide-based coating for AuNPs as drug carriers.

Main Methods:

• Synthesis and characterization of AuNPs using UV-VIS, DLS, and TEM.
• Confirmation of peptide capping on AuNPs via TEM and FTIR spectroscopy.
• Assessment of peptide stability using DSC and zeta potential measurements.

Main Results:

• Spherical AuNPs with an average diameter of 15 nm were successfully synthesized.
• Immobilization of the microalgae peptide onto AuNPs enhanced peptide stability, indicated by thermal shifts and high zeta potential.
• The resulting peptide-AuNP conjugates exhibited lower ecotoxicity and better viability compared to bare AuNPs.

Conclusions:

• Gold nanoparticles serve as effective carriers for microalgae peptides, enhancing their stability and functionality.
• The peptide-AuNP system presents a promising platform for drug delivery with improved biocompatibility.
• This study highlights the potential of microalgae peptides in nanomedicine and biomaterial development.