Polydatin Incorporated in Polycaprolactone Nanofibers Improves Osteogenic Differentiation

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Regenerative Medicine

Background:

• Polycaprolactone (PCL) nanofibers are utilized in tissue engineering for regeneration and drug delivery due to their biodegradability and clinical safety.
• Polydatin (PD), a precursor of resveratrol, exhibits antioxidant, antitumor, antiosteoporotic, and bone regeneration properties.

Purpose of the Study:

• To create an innovative, biomimetic scaffold (PCL-PD) leveraging polydatin's osteogenic capacity for bone tissue engineering.
• To evaluate the in vitro cytocompatibility and osteogenic potential of the PCL-PD scaffold using osteosarcoma cells (Saos-2) and mesenchymal stem cells (MSCs).

Main Methods:

• Fabrication of PCL-PD nanofibers for bone tissue engineering.
• In vitro assessment using MTT assay, scanning electron microscopy (SEM), and alkaline phosphatase (ALP) activity.
• Reverse-phase (RP) HPLC to determine polydatin release kinetics from the scaffold.

Main Results:

• MSCs proliferation on PCL-PD scaffolds was comparable to PCL scaffolds, while Saos-2 cell proliferation decreased.
• Significantly higher ALP activity was observed in both Saos-2 cells and MSCs cultured on PCL-PD scaffolds compared to controls after 14 days.
• SEM analysis revealed a spindle-shaped morphology in cells grown on PCL-PD scaffolds, indicating enhanced adhesion and differentiation.

Conclusions:

• The PCL-PD nanofiber scaffold demonstrates effective support for cell adhesion.
• The scaffold promotes osteogenic differentiation in both human osteosarcoma cells and MSCs, highlighting its potential in bone tissue engineering.