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Development of an Antibacterial Dentin Adhesive.
Stephanie R Lopes1, Amanda G N Matuda1, Raquel P Campos1
1Department of Restorative Dentistry, Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos 12245-000, São Paulo, Brazil.
Polymers
|June 24, 2022
Summary
Nisin-doped dental adhesives effectively inhibit Streptococcus mutans growth. While 1% nisin showed no impact on bond strength or conversion, higher concentrations reduced these properties.
Area of Science:
- Dental Materials Science
- Antimicrobial Peptides
- Cariology
Background:
- Dental caries remains a significant global health issue, primarily caused by Streptococcus mutans.
- Developing novel antimicrobial agents for dental adhesives is crucial for preventing secondary caries and improving restoration longevity.
- Nisin, a bacteriocin, exhibits broad-spectrum antibacterial activity and is a promising candidate for incorporation into dental materials.
Purpose of the Study:
- To evaluate the antibacterial efficacy of nisin-doped dental adhesives against Streptococcus mutans.
- To assess the impact of nisin incorporation on the degree of conversion and microtensile bond strength (μTBS) of a dental adhesive to dentin.
Main Methods:
- Nisin was incorporated into Adper Single Bond 2 at 1%, 3%, and 5% (wt%).
- Antibacterial activity was determined by colony-forming unit (CFU) counts against S. mutans.
- Degree of conversion was measured using Fourier-transform infrared spectroscopy (FTIR).
- Microtensile bond strength (μTBS) was evaluated on restored human teeth.
Main Results:
- All nisin concentrations significantly inhibited S. mutans growth (p < 0.05).
- Incorporation of 3% and 5% nisin significantly reduced the degree of conversion (p < 0.05).
- μTBS decreased with increasing nisin concentration: Control (38.3 ± 2.3 MPa), 1% (35.6 ± 2.1 MPa), 3% (27.1 ± 1.6 MPa), and 5% (22.3 ± 1.0 MPa).
- The 1% nisin group showed no significant difference in μTBS or degree of conversion compared to the control.
Conclusions:
- Nisin-doped adhesives demonstrate potent bactericidal effects against S. mutans.
- A 1% nisin concentration is optimal, preserving the adhesive's mechanical properties (degree of conversion and μTBS).
- Nisin offers a viable strategy for developing antimicrobial dental adhesives to combat cariogenic bacteria.
