Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Potentiometry: Membrane Electrodes

Potentiometry: Membrane Electrodes

Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...

Potentiometry: Overview

Potentiometry: Overview

Potentiometry is an analytical technique that measures the potential difference between two electrodes in an electrochemical cell without drawing any significant current that could alter the solution's composition. This method employs an indicator electrode, which exchanges electrons with the analyte solution, and a reference electrode with a constant potential. Each electrode is immersed in a solution comprised of two half-cells. In a conventional setup, the reference electrode serves as...

Potentiometry: Types of Electrodes

Potentiometry: Types of Electrodes

Reference electrodes serve as a stable reference point for potentiometric measurements, while indicator and working electrodes react to variations in the composition of a solution.
The Standard Hydrogen Electrode (SHE) is a widely used reference electrode that maintains zero potential across all temperatures. However, its need for a continuous hydrogen gas supply renders it impractical for everyday use.
An alternative to SHE is the Saturated Calomel Electrode (SCE). This electrode features an...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Control Circuits for Potentiostatic/Galvanostatic Polarization and Simultaneous Chemical Sensing by a Light-Addressable Potentiometric Sensor.

Sensors (Basel, Switzerland)·2024

Same author

Thermally Drawn CNT-Based Hybrid Nanocomposite Fiber for Electrochemical Sensing.

Biosensors·2022

Same author

Simultaneous In Situ Imaging of pH and Surface Roughening during the Progress of Crevice Corrosion of Stainless Steel.

Sensors (Basel, Switzerland)·2022

Same author

Novel anchoring device for endoscopic ultrasound-guided gallbladder drainage: Secondary publication.

Journal of hepato-biliary-pancreatic sciences·2022

Same author

Estimation of Potential Distribution during Crevice Corrosion through Analysis of <i>I-V</i> Curves Obtained by LAPS.

Sensors (Basel, Switzerland)·2020

Same author

Simulation and Experiment for Electrode Coverage Evaluation by Electrochemical Impedance Spectroscopy Using Parallel Facing Electrodes.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2020

Search research articles

Related Experiment Video

Updated: Sep 6, 2025

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Published on: September 27, 2019

Efficient Illumination for a Light-Addressable Potentiometric Sensor.

Tatsuo Yoshinobu¹, Ko-Ichiro Miyamoto²

¹Department of Biomedical Engineering, Tohoku University, Sendai 980-8579, Japan.

Sensors (Basel, Switzerland)

|June 24, 2022

Summary

Optimizing light-addressable potentiometric sensor (LAPS) performance requires efficient illumination. Square wave illumination with a low duty cycle doubles the signal output compared to sine waves for the same light power.

Keywords:

LAPS duty ratio field-effect device light-addressable potentiometric sensor lock-in detection modulated illumination pH sensor photocurrent square wave waveform

More Related Videos

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

Related Experiment Videos

Last Updated: Sep 6, 2025

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis

Published on: September 27, 2019

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Building a Simple and Versatile Illumination System for Optogenetic Experiments

Published on: January 12, 2021

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Local Field Fluorescence Microscopy: Imaging Cellular Signals in Intact Hearts

Published on: March 8, 2017

Area of Science:

Chemical Sensors
Semiconductor Devices
Photochemistry

Background:

Light-addressable potentiometric sensors (LAPS) utilize field-effect principles in an electrolyte-insulator-semiconductor structure.
Modulated illumination is crucial for LAPS to generate AC photocurrent signals responsive to ion activity.
High illumination power enhances signal-to-noise ratio but risks sample and measurement integrity.

Purpose of the Study:

To identify optimal modulated illumination waveforms for LAPS.
To maximize signal output for a given light input power.
To improve LAPS efficiency without compromising sample integrity.

Main Methods:

Investigated various modulated illumination waveforms for LAPS.
Compared signal generation efficiency across different waveforms.
Quantified photocurrent signal response to varying illumination patterns.

Main Results:

Square wave illumination with a low duty ratio significantly enhanced LAPS signal output.
The efficiency of square wave illumination was approximately double that of sine wave illumination.
Identified a more efficient method for LAPS signal generation.

Conclusions:

Square wave illumination, particularly with a low duty ratio, offers a superior method for LAPS signal enhancement.
This finding allows for improved LAPS sensitivity and performance.
Optimized illumination strategies are key to advancing LAPS technology.