Gene Editing and Genetic Control of Hemipteran Pests: Progress, Challenges and Perspectives

Area of Science:

• Entomology
• Genetics
• Agricultural Science

Background:

• The order Hemiptera originated over 230 million years ago, predating flowering plants.
• Phytophagous hemipterans possess specialized stylets for feeding on plant xylem and phloem, making them significant agricultural pests.
• Insecticide resistance in Hemiptera necessitates novel, species-specific, and eco-friendly pest control methods.

Purpose of the Study:

• To review progress in applying genetic control technologies to Hemiptera species.
• To discuss challenges and opportunities for gene editing in Hemiptera.
• To explore the potential of CRISPR/Cas9 for developing new pest management strategies in agriculture.

Main Methods:

• Review of recent advances in CRISPR/Cas9 gene editing in Hemiptera.
• Analysis of challenges in germline transformation for genetic control in Hemiptera.
• Assessment of successful gene editing in nine Hemiptera species over the past four years.

Main Results:

• Significant progress has been made in CRISPR/Cas9 gene editing across nine Hemiptera species.
• The primary challenge remains the efficient delivery of genetic material into the germline of Hemiptera.
• Successful gene editing in model insects suggests rapid advancement in Hemiptera if delivery issues are resolved.

Conclusions:

• CRISPR/Cas9 technology holds substantial potential for developing innovative genetic control strategies in Hemiptera.
• Overcoming germline transformation hurdles is crucial for realizing the full potential of gene editing in this insect order.
• Continued research is vital for extending contemporary genetic control methods to agriculturally important Hemiptera species.