Related Experiment Video
Updated: Sep 6, 2025

Sample Preparation to Bioinformatics Analysis of DNA Methylation: Association Strategy for Obesity and Related Trait Studies
Published on: May 6, 2022
METABOLOMIC ANALYSIS OF NORMAL WEIGHT, HEALTHY AND UNHEALTHY OBESITY: AMINO ACID CHANGE ACROSS THE SPECTRUM OF
A A Muresan1, A Rusu1, G Roman1
1"Iuliu Hatieganu" University of Medicine and Pharmacy - Diabetes, Nutrition and Metabolic Diseases, Cluj-Napoca, Romania.
Context:
Obesity is a complex and heterogeneous disorder with multiple phenotypes described. Although metabolomic biomarkers of obesity have been extensively studied, biomarkers of obesity phenotypes and differences between these phenotypes and normal-weight (NW) persons have been less investigated.
Objective:
The objective of this cross-sectional analysis was to investigate serum amino acids (AA) as markers of metabolic alterations in obesity phenotypes and NW.
Design:
Cross-sectional.
Subjects And Methods:
By targeted metabolomics we analyzed serum samples of 70 women using ultrahigh-performance liquid chromatography/mass spectrometry. Participants were divided into 3 groups: NW, metabolically healthy obesity (MHO) and metabolically unhealthy obesity (MUHO).
Results:
Five AAs were significantly different between study groups: cysteine, methionine, asparagine, glutamine, and lysine (p-value <0.05 and variable importance in the projection >1). Cysteine increased linearly with metabolic unwellness from NW to MUHO. Lysine and glutamine were significantly higher, and asparagine was significantly lower in NW and MHO than in MUHO.
Conclusions:
By trend and group analysis we identified specific changes in serum AAs along with the progression of metabolically unwellness.
More Related Videos
Related Concept Videos
Overview of Protein Metabolism
Amino acids play various roles in the body once they are absorbed into cells. They are restructured...
Regulation of Metabolism
Amino Acid Biosynthetic Pathways
Overview of Metabolism
Plant Metabolism
Sunlight, the primary source of energy in plants, is first absorbed by the chlorophyll pigments present in their leaves. Plants then use this energy to carry out photosynthesis, where water is oxidized into oxygen and carbon dioxide...

