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Updated: Jul 26, 2026
Visual Detection of Multiple Nucleic Acids in a Capillary Array
Published on: November 15, 2017
Recent advances in biological detection with rolling circle amplification: design strategy, biosensing mechanism, and
Yong-Ping Gao1,2, Ke-Jing Huang3, Fu-Ting Wang4
1International Joint Research Laboratory of New Energy Materials and Devices of Henan Province, School of Physics and Electronics, Henan University, Kaifeng, 475004, PR China. gqli1980@henu.edu.cn.
Abstract:
Rolling circle amplification (RCA) is a simple and isothermal DNA amplification technique that is used to generate thousands of repeating DNA sequences using circular templates under the catalysis of DNA polymerase. Compared to alternating temperature nucleic acid amplification such as polymerase chain reaction (PCR) amplification, RCA is more suitable for on-spot detection without the need for an expensive thermal cycler. In this study, the principle and classification of RCA are introduced, and the applications of RCA in the detection of pathogenic bacteria, nucleic acid tumor markers, viruses, and proteins are reviewed. Finally, the perspectives of RCA in biological detection are anticipated. The RCA method has a great potential for biological detection. This review aims to provide references for the further development and application of the RCA technique in biosensors.
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