Related Experiment Video
Updated: Sep 6, 2025

A Method for Measuring RNA N6-methyladenosine Modifications in Cells and Tissues
Published on: December 5, 2016
Mechanisms and Strategies for Determining m6 A RNA Modification Sites by Natural and Engineered m6 A Effector
1Institute for Chemical Research, Kyoto University Gokasho, Uji, Kyoto, 611-0011, Japan.
Abstract:
N6 -Methyladenosine (m6 A) is the most common internal RNA modification in the consensus sequence of 5'-RRACH-3'. The methyl mark is added by writer proteins (METTL3/METTL14 metyltransferase complex) and removed by eraser proteins (m6 A demethylases; FTO and ALKBH5). Recognition of this methyl mark by m6 A reader proteins leads to changes in RNA metabolism. How the writer and eraser proteins determine their targets is not well-understood, despite the importance of this information in understanding the regulatory mechanisms and physiological roles of m6 A. However, approaches for targeted manipulation of the methylation state at specific sites are being developed. In this review, I summarize the recent findings on the mechanisms of target identification of m6 A regulatory proteins, as well as recent approaches for targeted m6 A modifications.
Related Concept Videos
RNA Stability
RNA Editing
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...
Regulation of Expression at Multiple Steps
mRNA Stability and Gene Expression
Cis-acting Elements involved in mRNA stability
Nuclear Export of mRNA

