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Related Experiment Video

Updated: Sep 6, 2025

Author Spotlight: Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids
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Relief Effects of Icariin on Inflammation-Induced Decrease of Tight Junctions in Intestinal Epithelial Cells.

Yanli Li1, Jie Liu2, Pawin Pongkorpsakol3

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|June 27, 2022
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Summary

Icariin (ICA) protects the aging intestinal barrier by reducing inflammatory cytokines and restoring tight junctions. This involves decreasing miR-122a, offering a potential therapeutic strategy for gut health.

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ICAinflammatory cytokinesintestinal barrier functionmiR-122atight junction

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Area of Science:

  • Gastroenterology
  • Pharmacology
  • Cell Biology

Background:

  • Aging impairs intestinal barrier function due to inflammatory cytokines like TNF-α and IL-1β disrupting tight junctions.
  • Icariin (ICA) exhibits anti-inflammatory, anti-oxidative, and miRNA-modulating properties.
  • Network pharmacology predicted ICA's therapeutic potential for colitis, suggesting a role in gut health.

Purpose of the Study:

  • To investigate if Icariin (ICA) alleviates inflammation-induced intestinal barrier dysfunction in aging.
  • To elucidate the underlying molecular mechanisms of ICA's protective effects on intestinal barrier integrity.

Main Methods:

  • Network pharmacology was used for initial prediction of ICA's therapeutic targets.
  • Aging rats were treated with ICA to assess its effects on inflammatory cytokines and tight junctions.
  • Caco-2 cell monolayers were employed to explore ICA's mechanism in reversing TNF-α-induced tight junction disruption and permeability changes.
  • miR-122a mimic transfection was used to confirm its role in ICA's protective effects.

Main Results:

  • ICA intervention in aging rats reduced inflammatory cytokines (TNF-α, IL-1β) and increased tight junction proteins and antioxidant enzymes.
  • In Caco-2 cells, ICA reversed TNF-α-induced decrease in Occludin protein abundance and reduced epithelial permeability.
  • ICA decreased miR-122a expression, and its protective effect was diminished by miR-122a mimics, indicating miR-122a's crucial role.

Conclusions:

  • Icariin (ICA) reduces colon expressions of Occludin, Claudin1, and Claudin5 by decreasing TNF-α and IL-1β, alleviating colonic inflammation in vivo.
  • ICA protects against TNF-α-induced epithelial barrier impairment by down-regulating miR-122a expression in Caco-2 cell models.
  • ICA demonstrates potential as a therapeutic agent for age-related intestinal barrier dysfunction and colitis.