Dual fluorescent labeling of GLP-1R in live cells via enzymatic tagging and bioorthogonal chemistry
Tracey M Lewandowski1, Peng An1, Carlo P Ramil1
1Department of Chemistry, State University of New York at Buffalo Buffalo New York 14260-3000 USA qinglin@buffalo.edu.
RSC Chemical Biology
|June 27, 2022
Abstract:
To study GPCR conformational dynamics in live cells, here we report an integrated approach combining enzymatic SNAP-tagging with bioorthogonal chemistry for dual fluorescent labeling of GLP-1R. The resulting GLP-1R conformational biosensors permit a FRET-based analysis of the receptor subdomain movement in response to ligand stimulation in live cells.
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