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MicroRNA-877-5p Inhibits Cell Progression by Targeting FOXM1 in Lung Cancer

Zhiguang Liu1, Xinlian Wang2, Liqiang Cao3

  • 1Department of Respiratory and Critical Care Medicine, Changzhou Second People's Hospital Affiliated to Nanjing Medical University, Changzhou 213164, Jiangsu, China.

Canadian Respiratory Journal
|June 27, 2022
PubMed
Abstract

Insights

MicroRNA-877-5p acts as a tumor suppressor, inhibiting non-small-cell lung cancer (NSCLC) progression by targeting FOXM1. This finding offers potential biomarkers for NSCLC targeted therapy.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are recognized for their roles as oncogenes or tumor suppressors.
  • miR-877-5p functions as an antioncogene and is frequently downregulated in various cancers.

Purpose of the Study:

  • To investigate the biological functions and underlying mechanisms of miR-877-5p in non-small-cell lung cancer (NSCLC).
  • To identify potential therapeutic targets for NSCLC treatment.

Main Methods:

  • Bioinformatics tools were used to predict downstream targets of miR-877-5p.
  • Quantitative reverse transcription PCR (RT-qPCR) and Western blot assays were performed to analyze gene expression levels.
  • Cellular function assays were conducted to assess the effects of miR-877-5p and FOXM1.

Main Results:

  • miR-877-5p expression was found to be reduced in NSCLC tissues.
  • Overexpression of miR-877-5p inhibited the proliferation of NSCLC cells in vitro.
  • Upregulation of Forkhead box M1 (FOXM1) expression reversed the inhibitory effects of miR-877-5p.

Conclusions:

  • miR-877-5p suppresses NSCLC cell progression through direct targeting of FOXM1.
  • miR-877-5p serves as a potential biomarker for targeted therapy in NSCLC.

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