PHYSIOLOGICAL AND HISTOLOGICAL STUDY OF THE CALCIUM OXIDE NANOPARTICLES EFFECT ON THE TESTIS OF MALE WISTER RATS

The aim: The study carried out to determine the effect of the Calcium Oxide Nano Particles on the male rats. In this study were used calcium oxide nano powder to enhancing apoptosis in germ cells and disrupting hormonal regulation of reproductive processes in the adult male rats.

Materials and methods: The experiment using nine male rats, were distributed into three groups. Group one A was doses orally of Calcium Oxide Nano Particles 50 mg/kg of body weight, while group two B was doses orally of Calcium Oxide Nano Particles 100 mg/kg of body weight; also, group three C were a control C group treated with 0.9% saline only, these orally doses continuous 10 days. After 10 days blood samples collected and all rats were euthanatized and the weights, histological changes and hormonal analysis were conducted.

Results: Results show a significantly increased at p<0.05 for both groups A & B compare with control C in weight of testis tissue and the level of testosterone hormone, also histology changes in testis of treatment rats include: necrosis of spermatogonia, primary spermoocyte and spermatids in both groups compared to control group.

Conclusions: Through the study, it is recommended to use medium or low doses of CaO NPs that can be used as a testicular tonic and urge it to increase production of the Testosterone hormone.