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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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Related Experiment Video

Updated: Sep 6, 2025

Fluorescence Imaging with One-nanometer Accuracy FIONA

Fluorescence Imaging with One-nanometer Accuracy FIONA

Published on: September 26, 2014

Fluorescent Nanoparticles for Super-Resolution Imaging.

Wei Li^1,2, Gabriele S Kaminski Schierle², Bingfu Lei¹

¹Key Laboratory for Biobased Materials and Energy of Ministry of Education, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, People's Republic of China.

Chemical Reviews

|June 27, 2022

Summary

This summary is machine-generated.

Fluorescent nanoparticles offer bright, photostable probes for super-resolution microscopy, enabling nanometric visualization of cellular structures. This review details their use in advanced imaging and future material science opportunities.

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Last Updated: Sep 6, 2025

Fluorescence Imaging with One-nanometer Accuracy FIONA

Fluorescence Imaging with One-nanometer Accuracy FIONA

Published on: September 26, 2014

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

Area of Science:

Biophysics
Materials Science
Cell Biology

Background:

Super-resolution microscopy overcomes light diffraction limits for nanoscale cellular imaging.
Development of advanced fluorescent probes is crucial for enhancing imaging capabilities.
Fluorescent nanoparticles (NPs) are emerging as superior probes due to brightness and photostability.

Purpose of the Study:

To review the application of fluorescent nanoparticles in super-resolution imaging.
To discuss the demands of various super-resolution techniques on NP properties.
To explore the potential of material science in developing novel NPs for multiplexed imaging.

Main Methods:

Overview of super-resolution imaging techniques.
Detailed review of different fluorescent nanoparticle classes.
Analysis of NP features, strengths, and weaknesses for super-resolution applications.

Main Results:

Fluorescent NPs address limitations of traditional probes in super-resolution microscopy.
Specific NP types are suited for different super-resolution methods.
Examples of NP utilization in diverse biological systems are provided.

Conclusions:

Fluorescent nanoparticles are valuable tools for advanced cellular imaging.
Material science innovations are key to developing next-generation probes.
Future opportunities lie in multiplexed subcellular imaging with nanometric resolution.