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Aptamer-based biosensing through the mapping of encoding upconversion nanoparticles for sensitive CEA detection.

Zihui Xu1,2, Chunnan Wang1, Rui Ma1

  • 1Institute of Biopharmaceutical and Healthcare Engineering, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China. sun.shuqing@sz.tsinghua.edu.cn.

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Summary
This summary is machine-generated.

A novel aptamer-based biosensor system was developed for sensitive detection of carcinoembryonic antigen (CEA). This method accurately quantifies CEA levels, offering promise for early cancer diagnosis.

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Carcinoembryonic antigen (CEA) is a significant biomarker for various cancers.
  • Sensitive and accurate detection of CEA is crucial for early cancer diagnosis and monitoring.
  • Existing detection methods may lack the required sensitivity or simplicity.

Purpose of the Study:

  • To develop a novel aptamer-based biosensor for sensitive and accurate detection of CEA.
  • To utilize upconversion nanoparticles (UCNPs) for signal amplification and visualization.
  • To establish a quantifiable method for CEA determination in solution.

Main Methods:

  • Construction of a sandwich-structured probe using CEA aptamer-functionalized magnetic beads (MBs) and complementary DNA-modified UCNPs (cDNA-UCNPs).
  • Displacement of cDNA-UCNPs from MBs upon binding of CEA to the aptamer.
  • Quantification of CEA by counting target-dependent UCNPs using an inverted fluorescence microscope.

Main Results:

  • The developed biosensor demonstrated a sensitive detection of CEA.
  • A wide dynamic range for CEA detection was achieved (0.02-6.0 ng mL⁻¹).
  • A low limit of detection (LOD) of 65 fM was obtained, indicating high sensitivity.

Conclusions:

  • The aptamer-based UCNP biosensor provides a sensitive and accurate method for CEA detection.
  • The system shows potential for early cancer diagnosis through biomarker detection.
  • This platform can be extended for the detection of other cancer biomarkers.