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Structure and Coordination Determination of Peptide-metal Complexes Using 1D and 2D 1H NMR

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Published on: December 16, 2013

Synthesis and structural characterization of copper-cuprizone complexes.

M Jake Pushie¹, Kelly L Summers², Kurt H Nienaber³

¹Department of Surgery, College of Medicine, University of Saskatchewan, 107 Wiggins Road, Saskatoon, Saskatchewan S7N 5E5, Canada. jake.pushie@usask.ca.

Dalton Transactions (Cambridge, England : 2003)

|June 29, 2022

Summary

This study reveals the copper coordination structures of cuprizone, identifying the blue complex as Cu(III) and the green complex as an extended Cu(II) oligomer. These findings advance understanding of copper dyshomeostasis and demyelination models.

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Last Updated: Sep 6, 2025

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Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Accumulation and Analysis of Cuprous Ions in a Copper Sulfate Plating Solution

Published on: March 20, 2019

Area of Science:

Inorganic Chemistry
Biochemistry
Neuroscience

Background:

Copper(II) coordination with bis(cyclohexanone)oxalyldihydrazone (cuprizone) forms colored complexes used in copper detection.
Cuprizone induces demyelination in mice, serving as a model for studying copper dyshomeostasis and neurological disorders.
Structural characterization of copper-cuprizone complexes has been limited due to poor solubility.

Purpose of the Study:

To structurally characterize the copper coordination properties of cuprizone in both aqueous and non-aqueous media.
To elucidate the distinct copper coordination structures responsible for the blue and green complexes formed with cuprizone.
To provide insights into the mechanisms of copper dyshomeostasis relevant to demyelination.

Main Methods:

Utilized a combination of mass spectrometry and X-ray absorption spectroscopy.
Employed computational chemistry to complement experimental data.
Investigated copper-cuprizone complexes formed under varying conditions (aqueous vs. non-aqueous, excess ligand).

Main Results:

The blue copper-cuprizone complex, formed in aqueous conditions with excess ligand, features Cu(III) coordinated by two hydrolyzed cuprizone ligands.
The green copper-cuprizone complex, formed in non-aqueous media, is an extended oligomer with repeating Cu(II) centers bridged by unhydrolyzed cuprizone.
The Cu(II) centers in the green complex are positioned 4.8 Å apart.

Conclusions:

The study provides a self-consistent structural model for both blue and green copper-cuprizone complexes.
Understanding these structures is crucial for interpreting copper's role in cuprizone-induced demyelination.
This work clarifies the coordination chemistry of cuprizone, a key reagent in copper research and neurological models.