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Updated: Sep 6, 2025

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Processing human skin samples for single-cell assays.

Simona Saluzzo¹, Laura Marie Gail², Teresa Neuwirth³

¹Department of Dermatology, Medical University of Vienna, 1090 Vienna, Austria.

|June 30, 2022

Summary

This summary is machine-generated.

This study presents an optimized protocol for isolating viable human skin cells for single-cell analysis. The method enhances the study of immune cells in skin, aiding research into infections, inflammation, and cancer.

Keywords:

Cell isolation Health Sciences Immunology RNAseq Sequencing Single Cell

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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Published on: August 25, 2018

Area of Science:

Immunology
Dermatology
Genomics

Background:

Resident immune cells in human skin play crucial roles in immune responses.
Understanding these cells is vital for research into skin infections, inflammation, and cancer.
Existing methods for isolating skin cells can be improved for single-cell analysis.

Purpose of the Study:

To describe an optimized protocol for the rapid isolation of viable cells from human skin punch-biopsies.
To demonstrate the utility of this protocol by coupling it with single-cell RNA sequencing (scRNA-seq) and single-cell T-cell receptor sequencing (scTCR-seq).
To enable detailed characterization of transcriptional profiles and clonotypes of skin-resident and circulating T cells.

Main Methods:

Optimized protocol for isolating viable cells from 6-mm human skin punch-biopsies.
Coupling of single-cell RNA sequencing (scRNA-seq) and single-cell T-cell receptor sequencing (scTCR-seq).
Analysis of transcriptional profiles and T-cell clonotypes in skin and blood samples.

Main Results:

Successful isolation of viable immune cells from human skin biopsies.
Detailed characterization of transcriptional profiles of skin-resident and circulating T cells.
Identification of T-cell clonotypes in both skin and blood, providing insights into T-cell populations.

Conclusions:

The optimized protocol facilitates the study of human skin immune cells using single-cell assays.
This method enhances understanding of T-cell populations in skin and blood.
The protocol is an improvement over existing methods and supports research in skin immunity, infections, inflammation, and cancer.