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The Three-Chamber Choice Behavioral Task using Zebrafish as a Model System
Published on: April 14, 2021
Protocol for an AlCl3-induced Alzheimer's model in zebrafish larvae with optimized pH and behavioral assessment
Zequan Lin1, Lufang Zou1, Wei Li1
1Key Laboratory of Aquacultural Resources and Utilization, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
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Alzheimer's disease (AD) is a progressive neurodegenerative disorder that impairs cognition and memory. Current protocols for establishing AD models in zebrafish (Danio rerio) larvae are limited and lack comprehensive detail. We present a refined zebrafish model, optimizing pH control of AlCl3 solutions and improving the behavioral assays to ensure a consistent AD-like phenotype. We describe steps for breeding embryos, testing behavior, selecting and treating larvae, and analyzing data. This protocol facilitates high-throughput screening of potential therapeutic drugs and genes for AD.
