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Updated: Sep 6, 2025
In situ Imaging of the Mouse Thymus Using 2-Photon Microscopy
Published on: January 25, 2008
Establishing a multiplex imaging panel to study T cell development in the thymus in mouse
Amr H Allam1, Sarah M Russell2
1Optical Sciences Centre, School of Science Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, Australia; Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC, 3084, Australia; Immune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC 3000 Australia.
Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).
Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).
