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Related Experiment Video

Updated: Sep 6, 2025

Author Spotlight: Exploring Cell Migration and Gene Roles in the Developing Brain
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Imaging neuronal migration and network activity in human forebrain assembloids.

Fikri Birey1, Sergiu P Pașca1

  • 1Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA; Stanford Brain Organogenesis, Wu Tsai Neurosciences Institute and Bio-X, Stanford University, Stanford, CA, USA.

STAR Protocols
|June 30, 2022
PubMed
Summary
This summary is machine-generated.

Human pluripotent stem cell-derived assembloids model early brain development. Protocols are presented to track neuron migration and network activity in these human brain organoids.

Keywords:
Cell BiologyNeuroscienceOrganoids

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Area of Science:

  • Neuroscience
  • Developmental Biology
  • Stem Cell Biology

Background:

  • Assembloids, derived from human pluripotent stem cells, offer self-organizing in vitro models.
  • These models recapitulate key aspects of neural development, including cell-cell interactions and circuit assembly.

Purpose of the Study:

  • To present protocols for functional monitoring of forebrain assembloids.
  • To specifically track GABAergic interneuron migration and early cortical network activity.

Main Methods:

  • High-resolution imaging and analysis of neuronal migration.
  • Calcium imaging techniques to assess network activity in forebrain assembloids.

Main Results:

  • Detailed protocols for observing neuronal migration patterns.
  • Methods for analyzing network activity in developing cortical organoids.

Conclusions:

  • Established protocols enable functional monitoring of human brain organoids.
  • These methods advance the study of neural development and circuit formation in vitro.