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Updated: Sep 6, 2025
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
Published on: September 30, 2019
Selection and identification of a specific peptide binding to ovarian cancer cells from a phage-displayed peptide
Qian Gao1, Lirong Chen1, Chenshuang Jia1
1College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, Shaanxi, China.
Objectives:
Ovarian cancer is one of the most fatal gynecological malignancies. It is emergently needed to select a novel molecular fragment as a targeting element for the future development of molecular imaging diagnosis and targeting chemotherapy to ovarian cancer.
Results:
After five rounds of biopanning, a total of 44 positive phage clones were selected from final phage displayed peptide library. Nine consensus sequences were found based on the assay of sequencing results, then one clone of each consensus group was characterized and identified further by immunofluorescence assay. The result showed the phage clone R20 presents best targeting capacity. Then we synthesized peptide (OSP2) clone R20 displayed, it was characterized with high specificity and sensitivity binding to human ovarian cancer by a tissue chip assay. The target of OSP2 was predicted and docked as human carbonic anhydrase XII (CA12), an important protein usually deregulated in cancer.
Conclusions:
Taken together, OSP2 and its target indicate a novel investigation way in future to develop novel agent or drug delivery formulation for molecular imaging diagnosis and targeting chemotherapy of ovarian cancer.
Insights
Researchers identified a novel peptide, OSP2, that effectively targets ovarian cancer. This peptide shows promise for developing new molecular imaging and chemotherapy strategies for this fatal gynecological malignancy.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Ovarian cancer is a highly fatal gynecological malignancy.
- There is a critical need for novel targeting agents for diagnosis and therapy.
Purpose of the Study:
- To identify a novel molecular fragment for targeting ovarian cancer.
- To develop new agents for molecular imaging and chemotherapy.
Main Methods:
- Phage display biopanning was used to select targeting peptides.
- Immunofluorescence and tissue chip assays validated peptide binding.
- Bioinformatic tools predicted the peptide's target.
Main Results:
- Phage clone R20, displaying peptide OSP2, demonstrated significant targeting capacity for ovarian cancer.
- OSP2 showed high specificity and sensitivity in binding to ovarian cancer cells.
- Human carbonic anhydrase XII (CA12) was identified as the target of OSP2.
Conclusions:
- OSP2 represents a promising novel agent for ovarian cancer molecular imaging and targeted chemotherapy.
- The OSP2-CA12 interaction opens new avenues for therapeutic development.
- This research facilitates the creation of advanced drug delivery formulations.
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