Identification of Vasculogenic Mimicry in Histological Samples

Vasculogenic mimicry has been identified in several malignancies and is generally associated with aggressive tumor growth and increased risk of metastasis. Patterned matrix can be identified in light microscopy of tumor sections stained with periodic acid-Schiff (PAS) without hematoxylin counterstain. In this chapter, the process is comprehensively described including tissue sources, formalin fixation and paraffin embedding, staining protocols, and the method for pattern identification in the microscope. Specific pattern types are illustrated in figures, and a number of pitfalls are detailed. The text can be used as a guideline by any researcher or clinician that wishes to evaluate histological samples for the presence of vasculogenic mimicry.